Multiple Stationary Phase Matrix and Uses Thereof To Resolve Mixtures of Chiral Molecules
Application: Separation matrices that are used in combination to separate molecules on the basis of chirality and general physio-chemical properties of the molecule of interest.
Separation techniques are widely used in the biological, chemical, and pharmaceutical industries to selectively isolate different compounds. Most separation technologies rely on one type of interaction between a molecule of interest and a stationary phase comprising a functional modality, allowing interaction between the molecule of interest and the stationary phase via hydrophobic, aromatic, hydrophilic, cationic, anionic, or stereochemical interactions. However, many important molecules, and particularly the active pharmaceutical ingredient in pharmaceuticals (e.g., Lipitor, Zocor, Plavix, and Nexium) must be separated from their less-desired, ineffective, or hazardous enantiomers. Typically, adequate resolution of the two enantiomers of a chiral compound is only achieved through the use of other types of separation technologies in combination with a chiral based separation technology. As such, the separation and isolation of a single anantiomer is often a highly expensive and time-consuming undertaking. Therefore, there is a need for a single separation technology that can inexpensively and swiftly resolve mixtures of molecules on the basis of more than one type of reaction.
The present technology provides a multiple phase matrix to separate and isolate molecules of interest from complex mixtures for identification and quantitative purposes. The matrix allows resolution of diastereomers of a class of molecules and also separation of different classes of molecules.
Applications range across a broad spectrum of biology, medicine, and industrial applications wherein complex mixtures of achiral and chiral isomers is common. The proposed matrix utilizes the strengths of both achrial and chiral stationary matrices to resolve molecules based on multiple physical properties, thereby making it more effective and thus expanding the utility of the system for different types of mixtures. Through the use of this unique matrix, only a single chromatographic system is required to provide the resolution of molecules, thereby providing a simpler, better recoveries and more efficient method for resolving complex mixtures of molecules.
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