This invention involves the development of assay kit(s) that can quantify the level of specific acetyltransferases. The method included in this invention has the potential to lead to the development of additional kits that are capable analyzing different proteins that are acylated as part of their biology.
The utility of this invention is related to analytical methods to monitor many of the new cancer therapeutic drugs that involve deacetylation and deacylation inhibitors. These studies and therapy could benefit from the methods disclosed in this invention.
Additionally, research is focused on creating clinical diagnostic kits that demonstrate the specificity in cell extracts to provide signatures with predictive value of disease stratification and therapy.
A major advantage is the ease of using the components of this kit. One would only need access to a mass spectrometric facility to obtain the final readout.
Reference: Molecular Cell 24, 785 – 796, December 08, 2006