Warning: in_array() expects parameter 2 to be array, null given in /home/pharmalicensing/public_html/detail.php on line 234

Warning: in_array() expects parameter 2 to be array, null given in /home/pharmalicensing/public_html/inc_stats.php on line 82

Warning: array_push() expects parameter 1 to be array, null given in /home/pharmalicensing/public_html/inc_stats.php on line 85
Pharmalicensing | Life Science's Global Technology Marketplace
Save this technology
close
Save to Existing Project
Save to a New Project
High Throughput Determination of RNA Secondary Structure
Yeda R&D Co. Ltd Israel flag Israel
Abstract ID:
A novel genome-scale method for measuring RNA structure. RNA structure plays an important role in many biological processes. For genes that code for proteins, there is growing evidence that substantial regulation of their gene expression occurs......
Contact Tal Shapira, m.sc.
Participants
You
Email me a copy of this message

A novel genome-scale method for measuring RNA structure. RNA structure plays an important role in many biological processes. For genes that code for proteins, there is growing evidence that substantial regulation of their gene expression occurs post-transcriptionally. Much of this post-transcriptional regulation is thought to be mediated through secondary structure elements that are recognized by specific RNA binding proteins within the cell. The structures of RNA molecules are often important for their function and regulation, yet there are no experimental techniques for genome-scale measurement of RNA structure. Existing experimental methods for measuring RNA structure can only probe a single RNA structure per experiment and are typically limited in the length of the probed RNA. The outlined technology, termed parallel analysis of RNA structure (PARS), provides simultaneous in vitro profiling of the secondary structure of thousands of RNA species at single nucleotide resolution.


Applications






    • Determining the structure of entire transcriptomes of organisms





    • Systematic measurement of the effects of diverse conditions on RNA structure





Advantages






    • Suitable for high-throughput analysis, even in the presence of substantial amounts of biological and measurement noise





    • Recovers structural profiles with high accuracy and at nucleotide resolution





    • Readily applicable to many organisms





Technology's Essence


The outlined invention introduces PARS, a combined computational and experimental method for measuring the secondary structure of thousands of RNA species in parallel. It is based on deep sequencing fragments of RNAs that were treated with structure-specific enzymes. PARS was applied to profile the secondary structure of the messenger RNAs of the budding yeast Saccharomyces cerevisiae and obtain structural profiles for over 3,000 distinct transcripts.


Licensing Status

GO PREMIUM TO GET PATENT INFORMATION
FEATURED
Last Updated May 2016
Technology Type MECHANISM
Phase of Development EARLY STAGE
CORPORATION