The core technology of Kyungpook National University is to provide novel composition for glucose sensing comprising of nanofibrous membrane and method for manufacturing non-enzymatic glucose biosensor using the same.
Background and unmet needs: Most common technology for glucose analyzing in blood is enzyme-based method. In spite of impressive development in electro-chemical glucose biosensors, highly stable and reliable glucose-sensing devices had been needed. Enzyme-based glucose sensors have many problems such as instability of enzymes, oxygen-dependency and need of mediators. Furthermore, low or high humidity could be dangerous in using the sensors. Thus, there's a need for non-enzymetic glucose sensing material. Recently, present inventors proved the efficiency of non-enzymatic glucose sensing material based electrospinning nanofibrous. Nanofibrous showed excellent glucose sensing ability. However, method of directly collecting electro-radiated PVdF/PAPBA (poly (vinylidene floride) /poly (aminophenyl boronic acid)) –NFM on ITO has difficulties in controlling thickness and uniformity of the surface.
Discovery and Achievements: For more simple and efficient way of producing non-enzymetic glucose using electro-emitted PVdF/PAPBA-NFM, the present inventors scattered electro-emitted PVdF/PAPBA-NFM in a solvent such as acetone, and plated above solvents to eletrode.
The present technology provides composition for detecting glucose comprising of material to be obtained by scattering electro-emitted PVdF/PAPBA-NFM in the solvent. The solvent may contain further additives.
The composion can be used availably by non-enzymetic glucose biosensor, and prepared by using the PVdF/PAPBA as active material for glucose sensing, which is active material for glucose sensing and verified the effects in the sensibility, alternative and stability in the inventor' study before. More specifically, the PVdF/PAPBA may be contained at the rate of 90-99 wt% to 10-1 wt%. Also, because of using electrospun PVdF/PAPBA and having general caricteristics (for example, have high superficial area) of a nanofibrous membrane prepared by electro-irradiated process, the composition is profitable to application for biosensor.
The present technology provides further a preparation method for a electrospinning nanofibrous membrane, comprising the step of; (?) processing of preparing for nanofibrous membrane by electrospinning a mixture comprising of PVdF/PAPBA; (?) preparing of composition for detecting glucose by scattering electro-irradiated nanofibrous membrane obtained in step (?) into solusion; and (?) depositing composition for detecting glucose in step (?) on a electrode. The step (?) and (?) are a preparation process of the glucose-detecting composition which can be used directly through dissolved in a available solvent.
Key Features and Advantages:
(1) Excellent susceptibility, selectivity and stability
As the compounds of electro radiated PVdF/PAPBA maintain excellent susceptibility, selectivity and stability, they could be used in preparing biosensor efficiently.
(2) Providing simple and efficient methods for preparing a biosensor
The method for preparing glucose biosensor of the present invention made an outstanding improvement on conventional PVdF/PAPBA method which is complicated and tangled process.
(3) Excellent reproducibility and commercializability
The method of the present invention provide a production process of excellent reproducibility and commercializability and make it possible to prepare more delicate and suitable biosensor due to great susceptibility, selectivity and stability .
(4) IP Position
These technologies have been filed for a patent application in a multitude of countries including Korea. The pending patent applications are anticipated to be patented in the near future.