l Technology Platform
The core technology of Chung-Ang University is to provide G12 human rotaviruses having VP7 gene and VP4 gene; antibodies specific for the rotaviruses; and A vaccine composition comprising the same. The antibodies and the vaccine composition are effective for diagnosing rotavirus infection and for treatment of diseases caused by rotavirus.
l Background and unmet needs
Rotavirus is a major cause of diarrhea in infants and young children throughout the world. Center for Disease Control and Prevention has reported that every year 440,000 infants and young children under 5 years of age die due to rotavirus infection. Under this circumstance, WHO has declared that the development of an anti-rotaviras vaccine is a project of the highest priority. A multitue of approaches and researches have been made to prevent and treat diseases caused by the G12 human rotavirus but have finally failed to effectively treat them.
l Discovery and Achievements
VP7 gene and VP4 gene in the rotavirus constitute an outer capsid of the rotavirus and are associated with pathogenicity, immunogenicity, cellular adhesiveness and intrusion of the rotavirus. G serotype and P genotype of the rotavirus are classified based on VP7 gene and VP4 gene, respectively.
The result of seminested multiplex PCR analysis for VP7 gene and VP4 gene of the rotavirus obtained from the stool specimens of the children suffering from the rotavirus infection shows that G serotype and P genotype of the inventive rotavirus are G12 and P, respectively.
NSP4 gene, which plays an important role in the pathogenicity of the rotavirus, increases the level of cAMP or cGMP by binding to specific receptors which exist in the intestinal canal and cause diarrhea by activating cyclic nucleotide signaling pathway, inducing increased Cl- secretion and decreased absorption of Na+ and water.
Gene sequencing of NSP4 genotype of the inventive rotaviruses was conducted and it has been found that NSP4 genotype of the inventive rotaviruses is NSP4[B].
Ph.D Kim isolated and identified human rotavirus strains having the above-mentioned characteristics, which were named as CAU 195/G12 (KCTC 10988BP) and CAU 214/G12 (KCTC 10989BP).
As presented in Fig. 1a, the nucleotide sequences of V7 genes of CAU 195/G12 and CAU 214/G12 showed >90% identity with those of other G12 rotaviruses; 99.5% with each other; 98.6% and 99.2% with Se585 (AJ31174); and 89.5% and 90.3% with L26 (M58290). As also illustrated in Fig. 1b, VP7 genes of the subject rotaviruses showed the highest sequence homology with Se585 (AJ31174).