Cardiovascular disease is responsible for 30% death worldwide and hypertension remains to be the most treatable risk factor. Angiotensin-I-converting enzyme (ACE), being the enzyme responsible for regulating blood pressure in human body, was used in present work to test the potency of bioactive peptides generated from enzymatic proteolysis of winged bean seed. The resulting proteolysate demonstrated ACE-inhibitory and antioxidative activity, whereby ACE inhibition reached its highest activity, 78.5%, after 12 h proteolysis while antioxidative activities, determined using assays involving DPPH. radical scavenging activity and metal ion-chelating activity, reached peaks of 65.0% and 65.7% at 8 h and 14 h, respectively. These results indicate that winged bean seed proteolysate possess in vitro ACE inhibitory and antioxidative activities, enabling it to serve as potential ingredient to produce functional food with multiple biological benefits.
The present invention pertains to a process for manufacturing a proteolysate with antioxidant and ACE inhibitory properties, the isolate produced according the method of the invention, and peptides isolated therefrom. The products of the invention are particularly useful for the treatment of cardiovascular diseases and thus can be used as medicines or as food additives or supplements.